Cellular microparticles in diabetic nephropathy.
Describe your project. No more than 250 words: background, hypothesis, techniques/methods to be used, role of student.
Background: After cellular stimulation in the broad sense of the term, the cell releases submicrometric membrane fragments, termed microparticles (MP), to the extracellular environment. MP serve as vectors of cell-to- cell communication through the transfer of proteins, bioactive lipids and RNA to target cells. The role of MP in diabetic nephropathy (DN, a high prevalence condidition and the most common cause of end-stage renal disease requiring dialysis) is currently unknown.
Hypothesis: Since proximal tubular cells contribute largely to the development of DN, we hypothesize that when these cells are exposed to hypoxia and high-glucose concentration (these conditions resembling the actual diabetic milieu to which cells are exposed) they release MP that impair the normal cell response to hypoxia
Techniques to be used: Cell culture, Western blot, immunocytochemistry, work in hypoxia cabin, flow cytometry, cell transfection, RT-PCR
Role of the student and timeline: The role of the student is highly dependent on their laboratory experience. Therefore, we will adapt the project to the student’s experience and will develop a tailored training process for 4-5 weeks. Then, the student will perform at least one experiment and will analyze the data obtained.